RETENTION AND LIPOPHILICITY OF ESTRADIOL DERIVATIVES IN NORMAL- AND REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Authors

  • M. Ačanski University of Novi Sad, Faculty of Technology, 21000 Novi Sad, Serbia
  • Đ. Vujić University of Novi Sad, Faculty of Technology, 21000 Novi Sad, Serbia
  • Z. Nježić University of Novi Sad, Institute of Food Technology, 21000 Novi Sad, Serbia

Keywords:

estradiol derivatives, chromatography, normal phases, reversed phases, retention, lipophilicity

Abstract

The retention behaviour and separation ability of some estradiol derivatives were studied by HPLC on silica and C-18 commercially available columns. The mobile phases used were: benzene-ethyl acetate, benzene-tetrahydrofuran, benzene-acetonitrile, isopropanol-water and tetrahydrofuran-water in various proportions. The results are discussed in terms of nature of the solute, eluent and stationary phase. Correlation between the retention constants of estradiol derivatives obtained on C-18 column and log P calculated by program ACD log P was examined too.

Downloads

Published

2012-12-31

How to Cite

Ačanski, M. ., Vujić, Đ. ., & Nježić, Z. . (2012). RETENTION AND LIPOPHILICITY OF ESTRADIOL DERIVATIVES IN NORMAL- AND REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. Journal of Engineering &Amp; Processing Management, 4(1), 127–138. Retrieved from http://jepm2021.tfzv.ues.rs.ba/index.php/pub/article/view/75

Issue

Vol. 4 No. 1 (2012): Journal of Engineering & Processing Management

Section

Original scientific paper

License

Copyright (c) 2012 Journal of Engineering & Processing Management

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.